Flask Culture of Mycobacterium lepraemurium
نویسندگان
چکیده
منابع مشابه
Adherence of Mycobacterium lepraemurium to tissue culture cells.
Adherence of Mycobacterium lepraemurium to tissue culture cells was examined and compared with that of M microti. M lepraemurium used in the present study was maintained on 1% Ogawa egg yolk medium. This microbe adhered to HEp-2 cells much more than A31 or McCoy cells, though the adherence was unusually low as compared with the large infectious dose. Frequency distribution of the number of bact...
متن کاملRadiometric studies of Mycobacterium lepraemurium.
The radiometric method has been applied for studying the metabolism of M. lepraemurium and the conditions which might force or inhibit its metabolic activity in vitro. These organisms assimilate and oxidize (U-14C) glycerol, and (U-14C) acetate, but are unable to oxidize (U-14C) glucose, (U-14C) pyruvate, (U-14C) glycine and 14C-formate. When incubated at 30 degrees C M. lepraemurium oxidizes (...
متن کاملSuppression of immunity to Mycobacterium lepraemurium infection.
After injection of 10(8) live Mycobacterium lepraemurium (MLM) into the left hind footpad of mice, there is development of local swelling attributable to a granuloma of the cell-mediated immunity type. Concomitant intravenous inoculation of live MLM delays and may even suppress footpad swelling, the effects being proportional to the intravenous dose of organisms. Concomitant footpad infection a...
متن کاملComparative ultrastructure of Mycobacterium leprae and Mycobacterium lepraemurium cell envelopes.
The structural properties of the cell envelopes of Mycobacterium leprae and Mycobacterium lepraemurium were investigated by freeze-fracture, freeze-etching, and negative-staining techniques. Freeze-fracture split the cell wall and exposed the internal features of the peptidoglycolipid mycosidic filamentous network. The cell membrane was also split into two asymmetric faces. The external fractur...
متن کاملGrowth of Mycobacterium lepraemurium in Nonstimulated
Mycobacterium lepraemurium cells were found to multiply in normal mouse peritoneal-derived and bone marrow-derived macrophages in vitro. Whereas activated peritoneal-derived macrophages demonstrated marked bacteriostasis for M. lepraemurium, significant bactericidal activity was exhibited by activated bone marrow-derived macrophages. However, only a small proportion of the bacteria were killed ...
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ژورنال
عنوان ژورنال: Japanese journal of leprosy
سال: 1980
ISSN: 0386-3980,2185-1360
DOI: 10.5025/hansen1977.49.172